PRS Atoll

Atoll GmbH Product Summary:

Atoll GmbH have developed a unique range of purpose-designed Chromatography Columns, MediaScout®, to meet the emerging needs of biopurification and sample handling.

Primary application areas include:

(scroll down for details and links)

Resin Screening
Methods Scouting
Process Validation and Scale Down Modelling
Protein Drug Discovery
High Throughput Chromatography
Parallel Chromatography
Disposable Process Columns
Accurate and Reproducible 96-Array Resin Dispensing

Resin Screening and Methods Development:

Process development increasingly involves use of chromatography in Downstream Processing. The ability to compare the many available resins can be made more valid and more comprehensive with specially designed tools.

96-array Format MiniColumns:

Comparison of many different resins in parallel is best handled with fully automated robotic fluid handling, and for this Atoll has been first to develop RoboColumns® in a 96-array.

It is possible however to perform similar work with arrays in a centrifuge or manually by pipette, for which Atoll have developed similar small arrays of CentriColumns and PipetColumns.

Individual Small Scale MiniChrom columns for LC operation:

It is also useful to compare resins with a traditional LC system such as Akta Explorer (GE Healthcare) or alternative HPLC systems. For this, Atoll have developed a complete range of individual columns with alternative geometries to suit specific needs of the user. They include columns to match the geometry of above MiniColumns (~200ul bed volume) and up to 10ml bed volume.

ALL MediaScout COLUMNS, including MiniColumns, can be packed with optimal compression and with ANY process resin above 10um particles, and with ANY resin material.

MiniChrom columns are available in 5mm dia, 8mm dia and 11.3mm dia formats up to 10cmL, and are made of injection moulded biocompatible polypropylene.

The use of Atoll MiniChrom prepacked columns confers a quality standard hardware for valid comparisons between resins of all types and from all resin providers.

Validation and Scale Down Modelling of Process Columns:

It is difficult to emulate performance of a large scale process column at small scale unless certain criteria are adhered to. The column must be precisely the same bed height, and to avoid scale down artefacts such as wall effects caused by packing inconsistencies and hardware constraints, the diameter must be considered with care. Home packed columns typically use lab scale adjustable columns designed for general lab use, and these were never purpose designed for validation or scaledown modelling. Despite these limitations many companies submit data for regulatory approval with such home packed laboratory scale columns.

Atoll have designed a simple cost effective, purpose designed column hardware system which they offer to pack with ANY process resin above 10um particle diameter, compression packed to emulate the process column.

ValiChrom columns are a cost effective way to provide assured quality data for any validation study or scale down modelling of process columns. They can also be used to confidently predict performance criteria for process columns during Process Development.

High Throughput and Parallel Chromatography:

Atoll have opened a door for precision parallel chromatography for users in many areas of application.

With ability to use Robotic Fluid Handling systems from many alternative system manufacturers, RoboColumns offer an unique enabling technology for High Throughput Purification by Chromatography in a 96-array parallel configuration.

Whilst most robotic systems currently offer a 8-channel parallel operation, a RoboColumn plate provides a basic platform which is limited only by the imagination of the user and the system hardware design.

Typical applications include:

High Throughput Desalting of samples for Mass Spec samples
Multiple sample processing with Metal Affinity adsorption/desorption
Any small scale high throughput operation with chromatography resins

Disposable Process Chromatography:

There is an increasing use of disposables in biotherapeutics production, and a move towards developing disposable technology for Downstream Processing. This development is driven by costs of cleaning validation, disposal and cost of hardware and labour. In the vaccine industry, it is becoming cost effective to use columns for limited or zero repeat use, however, until Atoll developed MaxiChrom disposable prepacked process columns, chromatography had been unable to provide a cost effective solution.

Atoll have developed a range of disposable plastics column hardware which they will pack with any process resin up to 300mm diameter and to 60cmL. After use, single or multiple cyclic reuse, these columns can be disposed of by incineration, and replaced with a newly packed unit. Customers can supply their own lots of resin if necessary. All resins are compression packed to manufacturer's specifications and are of course supplied with requisite Performance Certification.

MaxiChrom offers a truly cost-effective alternative to home packed costly non-disposable hardware whilst maintaining a quality packed bed consistent with the needs of the modern pharmaceutical industry, and the demands of the regulatory authorities.

Resin Sampling with High Precision:

It is extremely difficult to dispense repetitive accurate quantities of slurry suspensions such as chromatography resins using a pipette based system, due to settlement and clogging of system restrictions and tips etc.

Atoll have developed a vacuum manifold based alternative, ResiQuot which allows the user to dispense accurate volumes of any chromatography particulate material into the wells of a 96 array plate.

ResiQuot allows a user to dispense his own resin samples accurately and with precision into microtitre plates for adsorption studies and other batch based comparisons of potential process resin materials.


Why PRS? Customer Profile Suppliers Atoll BIA Separations NanoSight PRS History Contact

	Atoll GmbH...Convenience in Chromatography You are here > Atoll Atoll GmbH Product Summary: Atoll GmbH have developed a unique range of purpose-designed Chromatography Columns, MediaScout®, to meet the emerging needs of biopurification and sample handling. Primary application areas include: (scroll down for details and links) Resin Screening Methods Scouting Process Validation and Scale Down Modelling Protein Drug Discovery High Throughput Chromatography Parallel Chromatography Disposable Process Columns Accurate and Reproducible 96-Array Resin Dispensing Resin Screening and Methods Development: Process development increasingly involves use of chromatography in Downstream Processing. The ability to compare the many available resins can be made more valid and more comprehensive with specially designed tools. 96-array Format MiniColumns: Comparison of many different resins in parallel is best handled with fully automated robotic fluid handling, and for this Atoll has been first to develop RoboColumns® in a 96-array. It is possible however to perform similar work with arrays in a centrifuge or manually by pipette, for which Atoll have developed similar small arrays of CentriColumns and PipetColumns. Individual Small Scale MiniChrom columns for LC operation: It is also useful to compare resins with a traditional LC system such as Akta Explorer (GE Healthcare) or alternative HPLC systems. For this, Atoll have developed a complete range of individual columns with alternative geometries to suit specific needs of the user. They include columns to match the geometry of above MiniColumns (~200ul bed volume) and up to 10ml bed volume. ALL MediaScout COLUMNS, including MiniColumns, can be packed with optimal compression and with ANY process resin above 10um particles, and with ANY resin material. MiniChrom columns are available in 5mm dia, 8mm dia and 11.3mm dia formats up to 10cmL, and are made of injection moulded biocompatible polypropylene. The use of Atoll MiniChrom prepacked columns confers a quality standard hardware for valid comparisons between resins of all types and from all resin providers. Validation and Scale Down Modelling of Process Columns: It is difficult to emulate performance of a large scale process column at small scale unless certain criteria are adhered to. The column must be precisely the same bed height, and to avoid scale down artefacts such as wall effects caused by packing inconsistencies and hardware constraints, the diameter must be considered with care. Home packed columns typically use lab scale adjustable columns designed for general lab use, and these were never purpose designed for validation or scaledown modelling. Despite these limitations many companies submit data for regulatory approval with such home packed laboratory scale columns. Atoll have designed a simple cost effective, purpose designed column hardware system which they offer to pack with ANY process resin above 10um particle diameter, compression packed to emulate the process column. ValiChrom columns are a cost effective way to provide assured quality data for any validation study or scale down modelling of process columns. They can also be used to confidently predict performance criteria for process columns during Process Development. High Throughput and Parallel Chromatography: Atoll have opened a door for precision parallel chromatography for users in many areas of application. With ability to use Robotic Fluid Handling systems from many alternative system manufacturers, RoboColumns offer an unique enabling technology for High Throughput Purification by Chromatography in a 96-array parallel configuration. Whilst most robotic systems currently offer a 8-channel parallel operation, a RoboColumn plate provides a basic platform which is limited only by the imagination of the user and the system hardware design. Typical applications include: High Throughput Desalting of samples for Mass Spec samples Multiple sample processing with Metal Affinity adsorption/desorption Any small scale high throughput operation with chromatography resins Disposable Process Chromatography: There is an increasing use of disposables in biotherapeutics production, and a move towards developing disposable technology for Downstream Processing. This development is driven by costs of cleaning validation, disposal and cost of hardware and labour. In the vaccine industry, it is becoming cost effective to use columns for limited or zero repeat use, however, until Atoll developed MaxiChrom disposable prepacked process columns, chromatography had been unable to provide a cost effective solution. Atoll have developed a range of disposable plastics column hardware which they will pack with any process resin up to 300mm diameter and to 60cmL. After use, single or multiple cyclic reuse, these columns can be disposed of by incineration, and replaced with a newly packed unit. Customers can supply their own lots of resin if necessary. All resins are compression packed to manufacturer's specifications and are of course supplied with requisite Performance Certification. MaxiChrom offers a truly cost-effective alternative to home packed costly non-disposable hardware whilst maintaining a quality packed bed consistent with the needs of the modern pharmaceutical industry, and the demands of the regulatory authorities. Resin Sampling with High Precision: It is extremely difficult to dispense repetitive accurate quantities of slurry suspensions such as chromatography resins using a pipette based system, due to settlement and clogging of system restrictions and tips etc. Atoll have developed a vacuum manifold based alternative, ResiQuot which allows the user to dispense accurate volumes of any chromatography particulate material into the wells of a 96 array plate. ResiQuot allows a user to dispense his own resin samples accurately and with precision into microtitre plates for adsorption studies and other batch based comparisons of potential process resin materials.